BIO RAD WESTERN BLOT APPARATUS EBAY PLUS

Three 26.5 x 28 cm gels or 12 Criterion gels Specifications for Bio-Rad's tank blotting cells.ģ PROTEAN ® II xi, 6 Criterion, or 12 Mini-PROTEAN gels A cooling coil coupled to a refrigerated water recirculator provides temperature regulation. Adjustable plate electrodes provide a strong and uniform electrical field - up to three gel cassettes can be placed in the tank with the minimum electrode distance between them, increasing the field strength and transfer efficiency.

BIO RAD WESTERN BLOT APPARATUS EBAY PLUS

With a 28 x 26.5 cm blotting area, the Trans-Blot Plus cell has the capacity to transfer three large-format gels or multiple smaller format gels simultaneously in as little as 15–30 min. Temperature regulation can be achieved using the super cooling coil (included) and a refrigerated water recirculator (purchased separately). High-intensity transfers can be performed with the electrodes placed 4 cm apart, with a single gel holder cassette between them. Standard field transfers are performed with the electrodes placed 8 cm apart with this arrangement, all three of the gel holder cassettes can be used simultaneously. This system is best for transfer of large-format gels or multiple mini- or midi-format gels. The Trans-Blot cell accommodates three gel holder cassettes, each with a 16 x 20 cm blotting area. The Trans-Blot cell offers a choice of plate or wire electrodes and variable placement of the electrodes for either standard or high-intensity blotting options. Temperature regulation during transfer can be achieved through the use of a sealed ice-block or optimal cooling coil.

The Criterion Blotter can transfer up to two Criterion gels (15 x 9.4 cm) or four mini gels simultaneously in 30–60 min. A self-contained Bio-Ice™ cooling unit absorbs the heat generated during transfer. The Mini Trans-Blot cell can transfer up to two mini gels (10 x 7.5 cm) in an hour and is available either as a complete apparatus or as a module that uses the buffer tank and lid of the Mini-PROTEAN ® Tetra cell for operation. The Mini Trans-Blot cell and the Criterion Blotter accommodate mini- and midi-format gels, respectively. Mini Trans-Blot ® Cell and Criterion™ Blotter These cooling systems prevent temperature fluctuations and overheating during high-intensity, extended, or native protein transfers

Cooling mechanism - cooling systems consist of an ice block, a sealed ice unit, or a cooling coil that is coupled to an external cooling mechanism.

Plate electrodes offer greater field strength than wire electrodes but wire electrodes may be more economical and generate less heat

Electrodes - tank transfer systems use either plate or wire electrode cards.

Cassettes are made of non-conductive material and designed to permit unimpeded flow of current and buffer through the gel and membrane sandwich

Gel holder cassette - the gel and membrane sandwich is held together between two foam pad and filter paper sheets, and placed into the tank within a gel holder cassette.

Ports on the lid allow connection points for the electrodes and are energized using an external power supply On the inside, the tank has slots for placement of the electrode cards, gel holder cassettes, and cooling element.

Buffer tank and lid - the buffer tank and lid combine to fully enclose the inner chamber during electrophoresis.Tank transfer systems contain the following elements: Selection of the appropriate system is largely dictated by the gel format used for separation and the desired throughput. The tank transfer systems offered by Bio-Rad are described below, and their specifications are summarized in the table below.

Bio rad western blot apparatus ebay